Whole-genome amplification in double-digest RADseq results in adequate libraries but fewer sequenced loci
نویسندگان
چکیده
منابع مشابه
Amplification Biases and Consistent Recovery of Loci in a Double-Digest RAD-seq Protocol
A growing variety of "genotype-by-sequencing" (GBS) methods use restriction enzymes and high throughput DNA sequencing to generate data for a subset of genomic loci, allowing the simultaneous discovery and genotyping of thousands of polymorphisms in a set of multiplexed samples. We evaluated a "double-digest" restriction-site associated DNA sequencing (ddRAD-seq) protocol by 1) comparing result...
متن کاملRADcap: sequence capture of dual-digest RADseq libraries with identifiable duplicates and reduced missing data.
Molecular ecologists seek to genotype hundreds to thousands of loci from hundreds to thousands of individuals at minimal cost per sample. Current methods, such as restriction-site-associated DNA sequencing (RADseq) and sequence capture, are constrained by costs associated with inefficient use of sequencing data and sample preparation. Here, we introduce RADcap, an approach that combines the maj...
متن کاملAdapterama IV: Sequence Capture of Dual-digest RADseq Libraries with Identifiable Duplicates (RADcap)
2 2. CC-BY-ND 4.0 International license peer-reviewed) is the author/funder. It is made available under a The copyright holder for this preprint (which was not. Abstract: 2 4 Molecular ecologists seek to genotype hundreds to thousands of loci from hundreds to thousands 2 5 of individuals at minimal cost per sample. Current methods such as restriction site associated 2 6 DNA sequencing (RADseq) ...
متن کاملGenomePlex Whole-Genome Amplification.
INTRODUCTIONPCR-based whole-genome amplification (WGA) has the goal of generating microgram quantities of genome-representative DNA from picogram or nanogram amounts of starting material. This amplification should introduce little, or ideally no, representational bias. In contrast to other techniques for WGA, PCR-based methods are generally less affected by DNA quality and are more applicable t...
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ژورنال
عنوان ژورنال: PeerJ
سال: 2018
ISSN: 2167-8359
DOI: 10.7717/peerj.5089